Composite

Part:BBa_J43000:Design

Designed by: Lauren Beatty   Group: iGEM06_Mississippi   (2006-10-12)


J45503.Q04400.E0430


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We learned after testing that the tetR switch was an inverter. Also, the YFP is repressed with tetR regardless of hydrogen concentration. Therefore this composite part does not work very well as a detector of hydrogen concentration.

Source

All parts came from the registry.

References

The hybB promoter was used previously by the UCSF 2005 team.